DNA

Part:BBa_K1614021

Designed by: Daniel Heid   Group: iGEM15_Heidelberg   (2015-09-18)

Software-generated p53 aptamer

Notice: Functional DNA

This part is a sequence of a functional ssDNA. It is only active as single-stranded DNA. It can not be cloned into a plasmid. For use order it as a DNA oligo.


MAWS software generated aptamer for the tetramerization domain of p53.

This aptamer was designed by software, using minmization of relative entropy to identify an optimal DNA sequence to bind p53.

Usage and Biology

This aptamer is used in an AptaBody to detect p53 (BBa_K1614022) on a Western blot.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

[edit]
Categories
Parameters
None